br Funding sources The Lundbeck Foundation Denmark R A and

Funding sources The Lundbeck Foundation Denmark (R124-A11485 and R181-2014-3372) and Carlsberg Foundation Denmark (CF14-0561) are acknowledged for grants enabling the analysis (TBB grants). The overseas collaboration was supported by the Knud Hojgaard Foundation Denmark (j.nr. 45.462), the Oticon Foundation Denmark and the Otto Monsteds foundation Denmark (12-70-0953) (TBB grants). This investigation was supported by National Institutes of Health under NIAMSAR054099 (MMM) and AR050180 (MLW), and the Ruth L. Kirschstein National Research Service Award (F32 AR061186) (CMH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Acknowledgments
Specifications Table [please fill in right-hand column of the table below] Value of the data
1. Data Mosquitoes from the Anopheles gambiae s.l. complex (both M and S molecular forms of A. gambiae) are widespread in West Africa where they seasonally experienced the harsh desiccating conditions of the dry season. How these mosquitoes survive the dry season and are engaged in exponential Sephin1 growth as soon as the rainy season starts is still undefined and very controversial [2,3]. This ability to survive the dry season suggests high physiological plasticity in mosquitoes. In order to highlight the general physiological changes involved in mosquitoes at the onset of the dry season, we compared protein abundance and metabolic fingerprints in the two molecular forms of A. gambiae exposed to the climatic conditions of the rainy and the early dry season. The M and S molecular forms of A. gambiae were recently described as two different species, named Anopheles coluzzii and A. gambiae, respectively [4,5]. Here, we are still referring to the M and S molecular forms, as in our published paper (Hidalgoet al. [1]).
2. Experimental design Mosquitoes were reared from eggs to adults into four programmable climatic chambers (Sanyo MLR 315H, Sanyo Electric Co., Osaka, Japan). Two climatic chambers were used to reproduce the climatic conditions of the rainy season, and two others to reproduce those of the dry season. Climatic conditions were programmed using the temperature and humidity cycles recorded in south-western Burkina-Faso (11°23′N, 04°24′W) with a Vantage Pro2 weather monitoring station (Weatherlink; Davis Instruments, Hayward, CA, U.S.A.). Climatic conditions were recorded in August 2010 (rainy season) and December 2010 (onset of the dry season), then hourly averaged to design 12 step cycles required to programme as closely as possible the natural daily climatic variations inside the climatic chambers [6]. Conditions inside the climatic chambers were tightly monitored throughout experiments using MicroLog Pro monitors (EC750, Davis Instruments, Hayward, CA, U.S.A.).
3. Proteomic assays For each mosquito species (M and S molecular forms) and rearing conditions (rainy and dry season), proteomic samples consisted of a pool of 30 teneral (1h-old adult) female mosquitoes. Samples were sent to the proteomics department of Applied Biomics (http://www.appliedbiomics.com, Applied Biomics, Inc. Hayward, CA, USA) for proteomic assays as described below.
4. Metabolomic assays: amino acid and sugar concentrations Metabolomic fingerprinting was conducted on both 1h-old and 24h-old adult females. For each mosquito species (M and S molecular forms) and rearing conditions (rainy and dry season), metabolomic samples consisted of a pool of five–six female specimens, so that the dry mass of each sample reached at least 1mg. Five to nine replicates were used for the different experimental conditions.
Acknowledgements These assays were supported by the Agence Nationale de la Recherche through grant ANR-08-MIEN-006 to FS. The authors would like to thank Moundai Tchonfienet, Sougrinoma Zoungrana and Boubakar Nikiema (IRSS, Bobo-Dioulasso) for their help in mosquito rearing and sample preparation. Authors would like to thank the PRIDE team for their support.